2014 Nobel prize project

Anjali Prabhala, Chad Pierce, Varun Shankaran

Summary

  • Remade the optical microscope with fluorescent molecules.
  • They bypassed the limit for the maximum resolution of nanoscopy.
  • Nanoscopy visualized the pathway of individual molecules inside living cells.
  • 2 separate principles were awarded.
  • Stefan Hell made the stimulator emission depletion microscopy in 2000.
  • Two laser beams are utilized; One that stimulates fluorescent molecules to glow, another cancels out all fluorescent except for that in a nanometer- sized volume.
  • This is better than Abbe's limit.
  • Betzig and Moerner laid the foundation for individual molecules on and off.
  • Betzig and Moerner look at the same area many times and only let a few molecules glow at a time.
  • This yields an image at nanolevel.
  • This was very important because
  1. It made it possible for the scientists to study living cells in the finest molecule details
  2. This passed the physical limit of maximum resolution.
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How is the super resolution microscopy being used today? future impact?

  • It is being used to view cells and molecules even closer than a normal microscope.
  • Its a fluorescent based microscope that breaks the resolution limit letting you see things as small as 200 nm.
  • It will let us study the cells and molecules more closely
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