Enzyme Lab Effects of pH
By: Alex Rafael C. 1st
Question
What affect do enzyme concentration and pH balance have on enzyme activity?
Identification of Variables
The Independent variable is time, which is measured in seconds.
The Dependent variables are enzyme concentration and pH.
The Constant was what the enzymes were composed of.
Hypothesis
Any change in the amount of pH over a period of time will be dependent on the level of concentration in enzymes present.
Materials
LabQuest Enzyme Suspension
LabQuest app Four 18 x 150 mm test tubes
Vernier Gas Pressure Sensor Ice
rubber-stopper assembly pH Buffers
10 mL graduated cylinder Test Tube Rack
250 mL beaker of water Thermometer
Hydrogen Peroxide Four Disposable Pipettes
Styrofoam Cup Logger Pro
Methodology
For Enzyme Concentration:
1. Wear Goggles
2. Connect plastic tubing to the valve on Gas Pressure sensor.
3. Connect Gas pressure sensor to LabQuest and choose new from the file menu.
4. Add 2, then 3, then 4 drops to each separate test tube respectively.
5. Record data of enzyme activity using gas pressure sensor.
6. Graph Data
Conclusion
At a pH of 7, the enzyme was in its prime state. At a pH of 10, the enzyme denatured and the graph and stats began to decline.