Enzyme Lab Effects of pH

By: Alex Rafael C. 1st

Question

What affect do enzyme concentration and pH balance have on enzyme activity?

Identification of Variables

The Independent variable is time, which is measured in seconds.

The Dependent variables are enzyme concentration and pH.

The Constant was what the enzymes were composed of.

Hypothesis

Any change in the amount of pH over a period of time will be dependent on the level of concentration in enzymes present.

Materials

LabQuest Enzyme Suspension


LabQuest app Four 18 x 150 mm test tubes

Vernier Gas Pressure Sensor Ice

rubber-stopper assembly pH Buffers

10 mL graduated cylinder Test Tube Rack

250 mL beaker of water Thermometer

Hydrogen Peroxide Four Disposable Pipettes

Styrofoam Cup Logger Pro

Methodology

For Enzyme Concentration:

1. Wear Goggles

2. Connect plastic tubing to the valve on Gas Pressure sensor.

3. Connect Gas pressure sensor to LabQuest and choose new from the file menu.

4. Add 2, then 3, then 4 drops to each separate test tube respectively.

5. Record data of enzyme activity using gas pressure sensor.

6. Graph Data

Conclusion

At a pH of 7, the enzyme was in its prime state. At a pH of 10, the enzyme denatured and the graph and stats began to decline.