Fermentation Lab

By: MiKaela Stokes, Jaci Vest, & McKenzie Ivins

Problem:

I wonder what would happen if we used carbonated beverages to observe the effect of environment on yeast activities.

Hypothesis

If, we use coke then it will effect the yeast activities more than anything else.

The manipulated variable...

carbonated beverage or not

The range to be used...

Over a class period

Control Description:

The control is just an experiment with only water.

3 Constant Factors:

1. The 5 graphs of yeast.

2. The time it is exposed.

3. The same size beakers.

Materials:

Package of dry active yeast

250 mL beaker

250 mL flask

Sugar packets

Ruler or meter stick

String

Balloon

Warm water (110 degrees F)

Dr. Pepper

Coca Cola

Procedure (Manipulated):

1. Fill the beaker with 50 mL of warm tap water.

2. Add 1 package of yeast to the warm water and stir until it is in solution.

3. Carefully pour the yeast solution into the flask.

4. Immediately place the ballon on the top of the flask.

5. Using your string, measure the size of your balloon ever 3 minutes for 15 minutes.

6. Record Data & come to conclusion.

Procedure (control):

1. Fill the beaker with 100 mL of warm tap water.

2. Add 1 package of yeast to the warm water and stir until it is in solution.

3. Carefully pour the yeast solution into the flask.

4. Place balloon on the tip of flask immediately.

5. Using your string, measure the size of your balloon ever 3 minutes for 15 minutes.

6. Record Data & come to conclusion.

Data:

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Graph:

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Graph Explained:

On the x axis there is the growth of the balloon in cm.

On the y axis is the type of beverage and its growth every three minutes.

Light blue is the starting moment, red is after three minutes, yellow is after 6 minutes, green is after 9 minutes, purple is after 12 minutes, and teal is after 15 minutes.

Analysis:

Overall, we did not have any complications with our experiment. Everything turned out quite well, the only thing we would be interested in changing if we redid it was to let the drink and yeast sit longer than 15 minutes.

Conclusion:

In conclusion, our hypothesis was incorrect. Turns out, the Dr. Pepper showed the most growth, but only by a little bit. This was due to Coca Cola only having 39 grams sugar while Dr. Pepper contained 64 grams of sugar.