DNA Replication
Indiana Ludwig p.2 10/27/16
DNA replication and DNA polymerase:
DNA Replication def: Before mitosis (during late interphase), the cell duplicates its DNA through the process of replication.
Function of Both: As put above the function of DNA polymerase is to make new strands of DNA and proofread the each of the new strands of DNA during DNA replication. While the function of DNA replication is to duplicate the cells DNA.
DNA replication step by step:
2.The leading strand (which is the easier one to replicate) has a small piece of RNA ( which is called primer) attach to the 3 prime end (specifically where the beginning point of replication is).
3.DNA polymerase binds to the leading strand where the primer is and starts adding the complementary bases (A=T and G=C)to the ones on the leading strand. While multiple primers bind to the lagging strand, with each one several bases apart, and DNA polymerase adds the complementary bases to the ones on the lagging strand creating Okazaki fragments, as it is discontinuous unlike with the leading strand which is continuous.
4.Once both strands are formed, the primers are removed and replaced with the correct bases and DNA ligase connects the Okazaki fragments. Finally both sets of strands coil into the double helix shape.
Sources:
https://study-biology.wikispaces.com/DNA+Replication
https://pdb101.rcsb.org/motm/3
http://www.nature.com/scitable/definition/helicase-307
http://www.nature.com/scitable/content/dna-replication-of-the-leading-and-lagging-14668888
http://www.bio.miami.edu/dana/250/25008_4.html
Info:
Pearson Biology book, pg. G-10, 351, and 350.
http://biology.about.com/od/cellularprocesses/ss/DNA-Replication.htm