Recombinant DNA

Made by: Cierra, Michael, and Symeon

What is it?

Recombinant DNA is taking a piece of DNA and combining it with another strand of DNA. By doing so, scientists are able to create a new strand of DNA. The most common recombinant process involves combining the DNA of two different organisms.

Process

1. The gene is isolated

2. DNA is cleaved by an enzyme into fragments

3. The gene is inserted into plasmid

4. Plasmid is taken by a cell such as bacterium

5. Cells with gene are cloned

6. Cells make a protein product

7. Copies of protein are harvested

Big image

Importance

Below are some of the areas Recombinant DNA will have an impact on:

  • recombinant vaccines
  • prevention and cure of sickle cell anemia
  • prevention and cure of cystic fybrosis
  • production of clotting factors
  • production of insulin
  • production of recombinant pharmaceuticals
  • plants that produce their own insecticides

Examples

  1. mouse and human DNA
  2. human and bacterial DNA
  3. viral and bacterial DNA

Plasmids

molecules of DNA found in bacteria. (the genes from the recombinant DNA is inserted into a plasmid)