Aunt Elda Experiment
Was it the anesthesia or something else that killed Elda?
Beer's Law
Background Information
Purpose
Before you can get started you will need a few materials. Those materials include: graduated cylinder, computer, food coloring, water, pipet, cuvettes, colorimeter, and the Lab Pro Software.
The Steps to the Procedure
2. You need to calibrate the colorimeter. Prepare a Blank by filling a cuvette 3/4 of the way full with distilled water.
- Only grab the cuvettes by the top edge of the ribbed sides.
- Always position the cuvettes with their reference mark facing toward the white reference mark at the top of the cuvette slot on the colorimeter.
4. Collect the absorbance data for the standard solutions. Click "collect" at the top of the Logger Pro document.
5. Discard the cuvette contents as directed by your teacher. Fill the cuvette 3/4 full with the 40% sample. Place it in the colorimeter and close the lid. When the absorbance value stabilizes click "keep", type the 40% in the edit box, and press the enter button.
6. Repeat the steps until you have sampled all of the solutions. onec you have finished gathering your data, click stop.
7.Rinse your cuvettes with water well and dry as best as you can. Fill full with the unknown solution and place it in the colorimeter. read the absorbance value displayed in the meter. Once it is stabilized, record the value in your data chart.