Recombinant DNA

Colby Fecht; 04/04/16

What is recombinant DNA?

Recombinant DNA, or rDNA is the term used for two DNA strands that were artificially constructed.

Plasmids...

A plasmid is a genetic structure in a cell that can replicate chromosomes independently. Plasmids relate to rDNA because without them you do not have a cloning vector, a important part of creating rDNA.

How rDNA is created.

The process of creating rDNA is called molecular cloning. There are two commonly used ways of creating rDNA. The difference between these two ways is one (PCR), uses a test tube free of living cells, while the other creates it inside of a living cell.


The forming of rDNA requires a cloning vector, a DNA molecule that replicates inside of a living thing. Vectors can come from either viruses or plasmids. They contain small parts of DNA necessary for the creation of rDNA.


The choice of vector depends on three things, the choice of host organism, size of DNA, and whether or not the foreign DNA can be expressed. There are two main types of vectors. A plasmid and viral vector.


There are 7 actual steps of creating rDNA. However, 3 steps have to be done before anything further can begin. First, the DNA has to choose its host and cloning vector. Then begins the preparation of the vector DNA. The last step of preparation, is preparing the DNA to be cloned.


Once the three preparation steps have been completed, the creation of DNA will begin. First, the recombinant DNA gets introduced into the host organism. Then the selection of organisms containing recombinant DNA are selected by the vector. Lastly, a screening of clones with desired DNA inserts and biological properties are shown.

Steps in Recombinant DNA technology or rDNA technology

Recombinant DNA Technology

Recombinant DNA technology is any use of it in daily life. Recombinant DNA technology is important because without we wouldn't be able to help and do some of the things we do in daily life. There are numerous uses of rDNA technology, but here are just five. Recombinant chynosin, recombinant insulin, recombinant blood clotting, recombinant hepatitis B vaccine, and to diagnose HIV.

Controversy of Cloning

Scientists have used rDNA to successfully clone a living organism. The first successful cloning was of a tadpole in 1952. A while later in 1996, scientists were able to clone the first mammal, a sheep. There are rumors scientists have also cloned animals like cows, goats, and mice. I believe in the future, scientists will clone the first human. I think it would look like the person, but will it act like the person? There is no definite proof that a clone would actually be an exact copy of the intended person. One reason is because the clone has not been through the same experiences the real person did. So they may not know or act the same way when asked something.

Transgenic Animals

Transgenic Animals are animals whose genes have been deliberately altered by the transfer of a gene from one animal to another. The first genetically altered animals available to the public were mice in 1974. Recombinant DNA relates to the creation of transgenic animals, is that you have to either combine the rDNA or insert the rDNA into the new genome.
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