Colby Fecht; 04/04/16
How rDNA is created.
The forming of rDNA requires a cloning vector, a DNA molecule that replicates inside of a living thing. Vectors can come from either viruses or plasmids. They contain small parts of DNA necessary for the creation of rDNA.
The choice of vector depends on three things, the choice of host organism, size of DNA, and whether or not the foreign DNA can be expressed. There are two main types of vectors. A plasmid and viral vector.
There are 7 actual steps of creating rDNA. However, 3 steps have to be done before anything further can begin. First, the DNA has to choose its host and cloning vector. Then begins the preparation of the vector DNA. The last step of preparation, is preparing the DNA to be cloned.
Once the three preparation steps have been completed, the creation of DNA will begin. First, the recombinant DNA gets introduced into the host organism. Then the selection of organisms containing recombinant DNA are selected by the vector. Lastly, a screening of clones with desired DNA inserts and biological properties are shown.