Polymerase Chain Reaction
By: Amber, Aakshi, Noah
1. A piece of DNA is heated. This separates the two strands.
2. At each end of the original piece of DNA, a short piece of DNA that complements a portion of the sequence is added.
3. DNA polymerase copies the region between the primers. These copies serve as templates to make more copies.
PCR is done in a machine called the Thermal Cycler. It amplifies segments of DNA.
Droplet Digital PCR
Droplet Digital PCR technology is a digital PCR method utilizing a water-oil emulsion droplet system
In order for PCR to take place, the DNA strands need to be replicated.