BEER'S LAW LAB
By: Hunter Liles
UNDERSTANDING BEERS LAW
Concentration - The concentration of the anesthesia solution is determined by measuring its absorbance with the Colorimeter.
(When a graph of absorbance vs. concentration is plotted for the standard solutions, a direct relationship should result. The direct relationship between absorbance and concentration for a solution is known as Beer's Law.)
- First plug in the Colorimeter to the computer interface and use Logger Pro.
- Then fill the cuvettes with 3/4's full of the different solutions
- Holding the cuvette by the ribbed sides, place it into the slot of the Colorimeter.
- Calibrate the Colorimeter.
- Collect the data, then repeat until finished.
- Draw a graph and put a line of best fit on it.
(DONT forget to test the mystery solution)
This is the Colorimeter that is used during the lab. Make sure to plug this into your laptop and calibrate it.
The cuvettes are suppose to be filled 3/4 full of solution. Be careful putting the cuvettes in the calorimeter. Place the ribbed sides on edges and the clear side facing forward.
This is the solution that you fill the cuvettes' 3/4 full. The solutions are labeled on each of the beakers.
HOW TO MAKE A % SOLUTION
- Express the % of a solution as a decimal. Ex: 5% = 0.05
- Multiply the decimal by the total volume. Ex: 0.05 x 1000mL = 50mL (ethylene glycol needed)
- Subtract the volume of the solute from the total solution volume. Ex: 1000mL (total solution volume) - 50mL (ethylene glycol volume) = 950mL (water needed)
- Dissolve 50mL ethylene glycol in about 950mL of water. Finally, bring the final volume up to 1000mL of water.
50mL ethylene glycol / 1000mL solution x 100 = 5% ethylene glycol solution
2. Since Elda didn't die from the anesthesia she could have died from other multiple things. Elda could have died from old age during the surgery or from a human mistake.
3. Beer's Law helped me solve this case by allowing me to figure out the concentration and absorbance of a solution which helped me determine if Aunt Elda received too much anethesia.