Recombinant DNA

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What is recombinant DNA

Recombinant DNA is DNA that has been formed artificially by combining constituents from different organisms. Plasmid is a genetic structure in a cell that can replicate chromosomes typically a small circular DNA strand in the cytoplasm of a bacterium or protozoan. Recombinant DNA is made by putting some DNA in a vector then cutting a piece off that has a restriction enzyme and then ligate the DNA insert into the vector with DNA Ligase. The insert contains a selectable marker which allows for identification of recombinant molecules. An antibiotic is used so a cell without a vector dies when put with a antibiotic and the cell with the vector will live because it is resistant. The vector is inserted into a cell in a process called transformation. Recombinant DNA technology includes cloning which is make an organism that is exactly the same as another one another type of recombinant DNA technology is diagnostics which tells you what is wrong with you or in forensics what killed you. Some more examples are reverse genetics gene therapy and genomics. A transgenic animal is an animal that has a body from a different type of animal growing on it the first transgenic animals were mice they use rDNA to make the new body parts for the mouse

Recombinant DNA