2008 Noble Peace Prize

Green fluorescent proteins

GFP

What is the green fluorescent protein or GFP?

The green fluorescent protein gets its name from the bright green color it emits in the presence of ultraviolet light. It can make cells glow and help scientists track interactions between cells.


  • Osamu Shimomura began studying jellyfish in to determine what makes them glow. He found a protein (GFP) in these fish containing a chemical that absorbs and emits light called a chromophore.
  • Martin Chalfie wanted to use the GFP to track cell activity inside a roundworm. When he got E. Coli to reproduce GFP, he accidentally discovered GFP can be reproduced without any additional proteins.
  • Roger Y. Tsien was able to change the color of the GFP by adding various amino acids to different parts of the protein.
"The brainbow" experiment genetically modified mice to produce different colored proteins. This caused the brains of these mice to glow all different colors.
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Why is GFP important?

  • Certain structures cannot be viewed under a microscope so GFP can help scientists view these really tiny structures
  • The strong green color from the protein shows up under blue and ultraviolet light.
  • This protein can help show the development of Alzheimer's disease in the brain or the growth of cancer tumors.
  • Using this GFP, scientists can track a single protein and the process it goes through to observe these diseases and find a cure.
  • Today, GFP is a standard tool for researchers all over the world.
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