Anesthesia Lab (Beers Law)
Solve Aunt Eldas Death
What happened?
What do you need?
Cuvette
Colorimeter
Computer
Before You Start Know...
-Cuvettes should only be touched on the rippled sides near the top.
-There should bee no bubbles in any solution.
-Make sure you always face the clear sides of the cuvette to the front and back of the colorimeter.
-The cuvette should only be filled 3/4 th's of the way.
-Pour the solutions down the drain and rinse the cuvette thoroughly.
Now That You're ready
-Calibrate your colorimeter.
*Prepare a 0% cuvette, this means it has 0% solution.
-Collect the absorbance data.
*Press "Collect".
*Wipe the outside clean and place it in the colorimeter.
*Allow the absorbance to stabilize.
*Press "Keep".
*Press "Enter". (Your data pair should now be plotted on the graph.)
-Repeat these steps until all the samples have been tested and plotted.
-Once you are all done press "Stop".
Once You Have Collected All the Known Samples:
-Fill a cuvette with the unknown solution.
-Clean the sides of the cuvette, place it in the colorimeter, and close the lid.
-Notice the value in the Absorbance box,
*You do not need to press "Collect" because it should show you the absorbance level in the Absorbance box.
*Put its value in the Data and Clculations Table.
- And lastly pour all the solutions down the sink.
Finally Pressesing the Data
Pick a good regression model and select "Try Fit" to see if it is correct.
Press "Ok" when you found the correct model.
After the Lab
In Order to Make a Percent Solution....
So...
T(p)=f (that would be the amount of food coloring needed)
And
T-f=w (that would be the amount of water needed)