Anesthesia Lab (Beers Law)
Solve Aunt Eldas Death
What do you need?
You must hold these on the rippled edges and clear edges MUST stay clean. wipe them clean before you place them in the colorimeter.
The purpose of a colorimeter is to see how much light is absorbed by the water.
When the colorimeter is attached to the computer the computer analyzes the data and makes it into a graph.
Before You Start Know...
-Cuvettes should only be touched on the rippled sides near the top.
-There should bee no bubbles in any solution.
-Make sure you always face the clear sides of the cuvette to the front and back of the colorimeter.
-The cuvette should only be filled 3/4 th's of the way.
-Pour the solutions down the drain and rinse the cuvette thoroughly.
Now That You're ready
-Calibrate your colorimeter.
*Prepare a 0% cuvette, this means it has 0% solution.
-Collect the absorbance data.
*Wipe the outside clean and place it in the colorimeter.
*Allow the absorbance to stabilize.
*Press "Enter". (Your data pair should now be plotted on the graph.)
-Repeat these steps until all the samples have been tested and plotted.
-Once you are all done press "Stop".
Once You Have Collected All the Known Samples:
-Fill a cuvette with the unknown solution.
-Clean the sides of the cuvette, place it in the colorimeter, and close the lid.
-Notice the value in the Absorbance box,
*You do not need to press "Collect" because it should show you the absorbance level in the Absorbance box.
*Put its value in the Data and Clculations Table.
- And lastly pour all the solutions down the sink.
After the Lab
In Order to Make a Percent Solution....
T(p)=f (that would be the amount of food coloring needed)
T-f=w (that would be the amount of water needed)