# Solutions Lab

## Characteristics (functions ) of an anesthesia that are desirable. (What does Anesthesia do?)

It stops nerve cells from absorbing sodium ions from other nerve cells so the brain doesn't get the signal of pain.

## Characteristics (functions) of an anesthesia that are not desirable (dangerous)

Doctors don't know the full side effects if there is any. Some gas can be highly explosive if mixed with oxygen.

## Lets get started

In this lab we are supposed to find out the cause of Aunt Zelda's death. She had surgery and randomly died. They think its from giving her to much anesthesia. So they asked us (C.I.A.) to investigate how she died. We had to test the concentration of many solutions, and then test the solution that was given to Aunt Zelda to see if it was too strong. Scientists say it takes over 40% of the Anesthesia to kill a human.

## Definitions

Concentration - a measure of the amount of dissolved substance contained per unit of volume.

The Transmittance - is simply the fraction of light in the original beam that passes through the sample and reaches the detector. The remainder of the light, 1 - T, is the fraction of the light absorbed by the sample.

Absorbance - is a measure of the quantity of light absorbed by a sample

## Solution

A solution is a homogeneous mixture of two or more substances. A solution may exist in any phase.

## First Step of Lab

Our first step in the lab was to make all of the solutions.

## Step by step on how to make different percent solutions.

To make a 0 % solution in a 10 mL test tube, add 10 mL of water and 0 mL of Solvent

To make a 10 % solution, add 9 mL of water and 1 mL of solvent

To make a 20 % solution, add 8 mL of water and 2 mL of solvent

To make a 30 % solution, add 7 mL of water and 3 mL of solvent

To make a 40 % solution, add 6 mL of water and 4 mL of solvent

To make a 50 % solution, add 5 mL of water and 5 mL of solvent

To make a 60 % solution, add 4 mL of water and 6 mL of solvent

To make a 70 % solution, add 3 mL of water and 7 mL of solvent

To make a 80 % solution, add 2 mL of water and 8 mL of solvent

To make a 90 % solution, add 1 mL of water and 9 mL of solvent

To make a 100 % solution, add 0 mL of water and 10 mL of solvent

## Second Step

Put each Solution of Anesthesia into a Colorimeter. It calculates how much light is allowed through the solution. It gives you the concentration of the solution(tells how strong the anesthesia is).

## Graph of Lab

This is the final graph we got from the lab. Aunt Zelda's solution on the graph is the point connected to the text box shown above. Which shows that her Anesthesia is about 28% concentrated.

## Graph of Aunt Elda's solution

Cuvette Number----------------------- concentration(%)

1------------------------------------------------------- 0

2------------------------------------------------------ 10

3------------------------------------------------------ 20

4------------------------------------------------------ 30

5------------------------------------------------------- 40

6----------------------------------------------------- 50

Aunt Elda----------------------------------------- 28

## Conclusion

In conclusion to this lab, I believe, that the concentration of anesthesia given to Aunt Zelda was not to strong. It wasn't the doctors fault for the mixture of the solution. I used Beers law to find the concentration of the Anesthesia by putting different percent solutions in the graph, then put a line that fits all points. Next I put in the anesthesia given to Aunt Zelda and matched it on the line. It told me that the concentration was 28%. I think the cause of death was that she died from the poison inside her appendix.