Factors Affecting Enzyme Activity

By: Amanda Wells

Question

How can we measure pressure of different factors of Enzyme Activity?

Variables

Independent Variable: Degrees

Dependent Variable: Enzyme Activity

Constants: Time, Drops of Enzyme Concentrate

Hypothesis:

Explanatory Hypothesis: As enzyme action increases, reaction rate increases.

Prediction: If enzyme concentration increases, then reaction rate increases.

Null Hypothesis: As enzyme concentration increases, then the reaction rate will not be affected.

Alternative Hypothesis: As enzyme concentration increases, then reaction rate increases.

Temperature Hypothesis:

Explanatory Hypothesis: As temperature increases, the reaction rate increases.

Prediction: If temperature increases, then the reaction rate increases.

Null Hypothesis: As temperature increases, then the reaction rate will not be affected.

Alternative Hypothesis: As temperature increases, then the reaction rate increases.

Materials

LabQuest

LabQuest app

Vernier Gas Pressure Sensor

rubber- stopper assembly

10 mL graduated cylinder

250 mL beaker of water

3% H202

styrofoam cup to use as a water bath

enzyme suspension

four 18 x 150 mm test tubes

ice

test tube rack

thermometer (or temperature probe)

four disposable pipettes

Logger Pro (optional)

Goggles

Methodology

First, putting on we put on goggles, connecting the plastic tubing to the valve on the Gas Pressure Sensor. Then, connecting the Gas Pressure Sensor to LabQuest and choose New from the File menu. The sensor automatically identifies itself. Thoroughly clean the test tubes from the previous experiment. Label them T 0-5, T 20-25, T 30-35, T 50-55 and place them in the test tube rack. Adding 3 mL of 3.0% H2O2 and 3 mL of water to each test tube. Then tapping table, choosing the clear all data from the table menu, and tapping graph to display the graph.

Measure the enzyme activity at o-5 C- and prepare a water bath at a temperature in a range of 0-5 C by placing ice and water in a Styrofoam cup. Using a thermometer or a temperature probe, check that the temperature remains in the range throughout the test. Adding ice as the experiment progresses. Place the test tube T 0-5 in the cold water for 5 minutes so that it reaches a temperature in the 0-5 C range. Recording the actual temperature of the test-tube contents in the blank in Table 3. Then adding 2 drops of the enzyme solution to test-tube T 0-5. Repeating the steps above for each temperature test tubes.

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Conclusion

In conclusion, the measure of pressure of oxygen gas formed as H2O2 is 'destroyed,' from the enzyme catalyzing it. with different amounts of enzyme concentration and at different temperatures creates different amount of pressure.