Factors Affecting Enzyme Activity
By: Amanda Wells
Question
Variables
Dependent Variable: Enzyme Activity
Constants: Time, Drops of Enzyme Concentrate
Hypothesis:
Prediction: If enzyme concentration increases, then reaction rate increases.
Null Hypothesis: As enzyme concentration increases, then the reaction rate will not be affected.
Alternative Hypothesis: As enzyme concentration increases, then reaction rate increases.
Temperature Hypothesis:
Prediction: If temperature increases, then the reaction rate increases.
Null Hypothesis: As temperature increases, then the reaction rate will not be affected.
Alternative Hypothesis: As temperature increases, then the reaction rate increases.
Materials
LabQuest app
Vernier Gas Pressure Sensor
rubber- stopper assembly
10 mL graduated cylinder
250 mL beaker of water
3% H202
styrofoam cup to use as a water bath
enzyme suspension
four 18 x 150 mm test tubes
ice
test tube rack
thermometer (or temperature probe)
four disposable pipettes
Logger Pro (optional)
Goggles
Methodology
Measure the enzyme activity at o-5 C- and prepare a water bath at a temperature in a range of 0-5 C by placing ice and water in a Styrofoam cup. Using a thermometer or a temperature probe, check that the temperature remains in the range throughout the test. Adding ice as the experiment progresses. Place the test tube T 0-5 in the cold water for 5 minutes so that it reaches a temperature in the 0-5 C range. Recording the actual temperature of the test-tube contents in the blank in Table 3. Then adding 2 drops of the enzyme solution to test-tube T 0-5. Repeating the steps above for each temperature test tubes.