Beer's Law Lab
Beer's Law Lab
Pre-Lab Analysis of Anesthesia
Making a solution
Example: 10% solution
Get regular water for a base concentration
Get the green solution
Put 90 ml of water in a tube
Put 10 ml of green solution in a tube
That makes a 10% solution
Do the same thing for the other solutions just with different measurements of water and green solution to make any percent solution
Before Lab steps
First we made the solutions. We made them by mixing and measuring the water and the other liquid. Certain measurements were made. We had one with just regular water, a 10% solution, 20% solution, 30% solution, 40% solution, 50% solution, and a 60% solution. On top of the solutions we had the solution that the doctor used so we could compare that one with the graph and find out if Aunt Elda died from the anesthesia.
We placed all of our solutions in the Calorimeter. A Calorimeter monitors the light received by the photocell as either an absorbance or a percent transmittance. A higher concentration of the solution will absorb more light than a solution of lower concentration. The Calorimeter measures that. We measured all of our solutions. They all went up by 10% so on the graph it was your regular gradual line of increase. Then, we put the solution that the doctor used in the Calorimeter and it was about 33.8% which is below 40%. The anesthesia did not kill Aunt Elda.
Aunt Elda did not die from too much anesthesia because what the doctor used was below the lethal point of over 40%. We know that Aunt Elda died but it was not from the anesthetics.