Beer's Law Lab

Beer's Law Lab

Aunt Elda died while in surgery and we tried to figure out if it was the anesthesia that killed her. The anesthesia that the doctor used was left behind for us to use as a sample. A person can die from too much anesthesia. The amount that is lethal is over 40%. The normal amount of anesthesia used for putting patients down is less than 40%. The question is did Aunt Elda die from too much anesthesia.

Pre-Lab Analysis of Anesthesia

The experience that I have had with anesthesia is when I went to the dentist for my teeth. The anesthesia made me feel weird, dizzy, loopy, and numb. I couldn't feel things. It made feel good in a weird way. The desirable characteristics of anesthesia are how it makes you feel happy and laugh. It also makes things not hurt. The undesirable characteristics are how you feel dizzy and confused. Also, it they give you a too little amount of anesthesia, you could start to hurt and feel pain.

Making a solution

Example: 10% solution

Get regular water for a base concentration

Get the green solution

Put 90 ml of water in a tube

Put 10 ml of green solution in a tube

That makes a 10% solution

Do the same thing for the other solutions just with different measurements of water and green solution to make any percent solution

Before Lab steps

First we made the solutions. We made them by mixing and measuring the water and the other liquid. Certain measurements were made. We had one with just regular water, a 10% solution, 20% solution, 30% solution, 40% solution, 50% solution, and a 60% solution. On top of the solutions we had the solution that the doctor used so we could compare that one with the graph and find out if Aunt Elda died from the anesthesia.

Below are some of the materials we used in the lab.

Actual Lab

We placed all of our solutions in the Calorimeter. A Calorimeter monitors the light received by the photocell as either an absorbance or a percent transmittance. A higher concentration of the solution will absorb more light than a solution of lower concentration. The Calorimeter measures that. We measured all of our solutions. They all went up by 10% so on the graph it was your regular gradual line of increase. Then, we put the solution that the doctor used in the Calorimeter and it was about 33.8% which is below 40%. The anesthesia did not kill Aunt Elda.

The graph above is the one we made and used to compare the solutions.

Conclusion

Aunt Elda did not die from too much anesthesia because what the doctor used was below the lethal point of over 40%. We know that Aunt Elda died but it was not from the anesthetics.