Purpose of the lab:

Experiment with solutions to see how it relates to anesthesia with Aunt Elda, and to see if she survives.


Anesthesia was not used until the mid-1800s, therefore surgery was extremely painful. Even though it wasn't used until then, people in 4200 B.C. were discovering natural substances that could cause the loss of consciousness. Ever since then we have used anesthesia for surgeries a ton. It has saved many lives, and we would not be where we are today without it.

Prelab questions:

1. Tell me about an experience that you have had with anesthesia (dentist/doctor) If you have never had the experience, do you foresee a future experience?

I have had one surgery and multiple procedures done on my teeth using anesthesia. There could be more operations done on my teeth in the future.

2. What are characteristics {functions ) of an anesthesia that are desirable?

Not being awake and feeling pain during the operation. Some like the day dreams of laughing gas.

3. What are characteristics (functions) of an anesthesia that are not desirable (dangerous)?

Too much could kill you and too little could cause you to wake up or start feeling pain during procedure.

The lab

We tested 8 substances with different concentration percentages to see their absorbency levels. The right level to put someone down is 40%. Anything more than that could kill someone and anything less could cause them to wake up during the operation. We found that 40 and 50 percent concentration would be the best to use.

How to make a percent solution


colorimeter-see picture below left


computer with logger pro

1. Plug the colorimeter into your computer and pull up logger pro

2. Open Beer's Law program on logger pro

3. Get the solutions you'll use and place one in the colorimeter

4. Click collect on logger pro then click keep

5. Do this for all of the solutions and analyze your data


Cuvette Number- Concentration (%)

1- 0.2%

2- 6.5%

3 -15.6%

4- 28.4%

5- 42%

6- 49.8%

Aunt Elda- 55.9%


1. Was the concentration of Aunt Elda's anesthesia too strong?


2. If yes, how did that cause her death? If no, do you have a hypothesis for the cause of her death?

It was over the safe amount of concentration.

3. How did Beer's Law help you solve this case?

We used the materials to figure out concentration levels to see if they used a safe amount.

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